| 1. 25名活动性UC患者随机分为2组，12名口服益生菌（含有干酪乳杆菌Zhang、植物乳杆菌P-8、动物双歧杆菌乳亚种V9），13名口服安慰剂，2组患者同时服用相同剂量的美沙拉嗪，干预12周；|
2. 益生菌组患者的UC疾病活动指数（UCDAI）降低显著高于安慰剂组，缓解率也显著升高（91.67% vs. 69.23%）；
Microbial Biotechnology [IF:5.328]
Modulation of gut mucosal microbiota as a mechanism of probiotics‐based adjunctive therapy for ulcerative colitis
This was a pilot study aiming to evaluate the effects of probiotics as adjunctive treatment for ulcerative colitis (UC). Twenty‐five active patients with UC were assigned to the probiotic (n = 12) and placebo (n = 13) groups. The probiotic group received mesalazine (60 mg kg−1 day−1) and oral probiotics (containing Lactobacillus casei Zhang, Lactobacillus plantarum P‐8 and Bifidobacterium animalis subsp. lactis V9) twice daily for 12 weeks, while the placebo group received the same amounts of mesalazine and placebo. The clinical outcomes were assessed. The gut mucosal microbiota was profiled by PacBio single‐molecule, real‐time (SMRT) sequencing of the full‐length 16S rRNA of biopsy samples obtained by colonoscopy. A significantly greater magnitude of reduction was observed in the UC disease activity index (UCDAI) in the probiotic group compared with the placebo group (P = 0.043), accompanying by a higher remission rate (91.67% for probiotic‐receivers versus 69.23% for placebo‐receivers, P = 0.034). The probiotics could protect from diminishing of the microbiota diversity and richness. Moreover, the gut mucosal microbiota of the probiotic‐receivers had significantly more beneficial bacteria like Eubacterium ramulus (P < 0.05), Pediococcus pentosaceus (P < 0.05), Bacteroides fragilis (P = 0.02) and Weissella cibaria (P = 0.04). Additionally, the relative abundances of the beneficial bacteria correlated significantly but negatively with the UCDAI score, suggesting that the probiotics might alleviate UC symptoms by modulating the gut mucosal microbiota. Our research has provided new insights into the mechanism of symptom alleviation in UC by applying probiotic‐based adjunctive treatment.
Ping Chen,Haiyan Xu
XiaoFeng Zhou,Heping Zhang
Ping Chen,Haiyan Xu,Hai Tang,Feiyan Zhao,Chengcong Yang,Lai‐Yu Kwok,Chunli Cong,YanFang Wu,Wenyi Zhang,XiaoFeng Zhou,Heping Zhang